Sir M Visvesvaraya Institute Of Technology

BIOTECHNOLOGY DEPARTMENT

Tuesday, May 12, 2020

18BTL48- IMMUNOTECHNOLOGY LABORATORY

18BTL48- IMMUNOTECHNOLOGY LABORATORY

Sl No

Title of the Experiments

Sub-link

1.       

Agglutination Technique: ABO typing

http://vlab.amrita.edu/?sub=3&brch=69&sim=192&cnt=1

2.       

Differential counting of WBC

https://www.youtube.com/watch?v=tBZLiLT4g6s

3.       

Isolation of lymphocytes from peripheral blood

http://vlab.amrita.edu/?sub=3&brch=69&sim=1422&cnt=1

4.       

Bacterial Agglutination reaction-Widal test (Tube / slide agglutination)

https://www.youtube.com/watch?v=dflOQ8hXUbA

5.       

Radial Immunodiffusion (RID)

https://www.youtube.com/watch?v=ADtT2dA1lsc

6.       

Ouchterlony Double Diffusion (ODD)

http://vlab.amrita.edu/?sub=3&brch=70&sim=689&cnt=1

7.       

Rocket immunoelectrophoresis (RIEP)

https://www.youtube.com/watch?v=52opF5Cc1-k

8.       

Counter-current immunoelectrophoresis (CCIEP)

https://www.youtube.com/watch?v=w3NtwXOa2Ow

https://www.youtube.com/watch?v=D2O5OtMmzHU

9.       

Dot ELISA

http://vlab.amrita.edu/?sub=3&brch=69&sim=721&cnt=1

10.   

Coombs test

http://vlab.amrita.edu/?sub=3&brch=69&sim=195&cnt=1

11.   

Complement fixation test

https://www.youtube.com/watch?v=J-XEaBkbLZI

12.   

Isolation of Chicken Immunoglobulin (IgY) from Egg Yolk

http://vlab.amrita.edu/?sub=3&brch=69&sim=1419&cnt=1

13.   

Immunoglobulin purification from serum by IEC

http://vlab.amrita.edu/?sub=3&brch=70&sim=1099&cnt=1

14.   

Western blotting

https://www.youtube.com/watch?v=AnjBg587mGg

 

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