15BTL68- BIOKINETICS AND ENZYME TECHNOLOGY LABORATORY
SEMESTER –VI
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Laboratory Experiments: |
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A) Experiments based on
Kinetics |
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Mixed Flow Reactor |
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Plug Flow Reactor |
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Isothermal Batch Reactor |
https://www.youtube.com/watch?v=QiLv0SqbxCk |
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RTD in Mixed Flow Reactor |
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RTD in Plug Flow Reactor |
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B) Experiments based on Enzyme
Technology |
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Isolation of enzymes |
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Determination of specific activity of human
salivary α- amylase |
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Determination of Vmax & Km of human salivary
α- amylase |
https://www.youtube.com/watch?v=feibixFSTs0 |
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Effect of Inhibitor on human salivary α- amylase |
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Time course of Amylase activity |
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Effect of pH on human salivary α- amylase |
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Enzyme Immobilization techniques and kinetics |
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Isolation of papain from papaya and assay of
papain using calorimetric method |
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Effect of organic solvents on amylase activity |
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Determination of molecular weight by SDS-PAGE |
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